About this item
The HIM3-4 monoclonal antibody reacts with human CD33, a 67 kDa member of the sialoadhesion family. CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes. Hematopoietic stem cells and lymphocytes do not express this antigen. CD33 plays a role in sialic-acid dependent cell adhesion. Applications Reported: The HIM3-4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIM3-4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4 °C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
Flow Cytometry: 5 µL (1 µg)/test
Type: Primary
Antigen: CD33
Clonality: Monoclonal
Clone: HIM3-4
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Catalog No:
- 102049-130
- 102049-132
- Antigen Symbol:
- CD33
- CD33
- Antigen Name:
- CD33 molecule
- CD33 molecule
- Antigen Synonyms:
- SIGLEC-3|FLJ00391|p67
- SIGLEC-3|FLJ00391|p67
- Antibody Type:
- Primary
- Primary
- Clonality:
- Monoclonal
- Monoclonal
- Conjugation:
- PE (Phycoerythrin)-Cy5®
- Clone:
- HIM3-4
- HIM3-4
- Reactivity:
- Human
- Human
- Host:
- Mouse
- Mouse
- Gene ID:
- 945
- 945
- Isotype:
- IgG1 kappa
- IgG1 kappa
- Flow Cytometry:
- Yes
- Yes
- Form:
- Liquid
- Liquid
- Purification:
- Affinity chromatography
- Affinity chromatography
- Concentration:
- 5 µl/Test
- 5 µl/Test
- Storage Buffer:
- PBS, 0.1% gelatin, 0.2% BSA, 0.09% sodium azide, pH 7.2
- PBS, 0.2% BSA, 0.1% gelatin, 0.09% sodium azide, pH 7.2
- Storage Temperature:
- 4 °C, store in dark, Do not freeze!
- 4 °C, store in dark, Do not freeze!
- Tested Applications:
- Flow Cytometric Analysis
- Flow Cytometric Analysis