Corning® Trypsin
Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging. Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action. EDTA sequesters these ions and thus enhances the efficacy of trypsin.
Storage: -25°C to -15°C
Trypsin has a pH specification range from 7.2 to 8. At this range, phenol red inclusion gives the solution a pink color. During shipping, the trypsin might change color from pink to yellow due to exposure to carbon dioxide released from the dry ice in the shipping container. In our experience, this discoloration does not affect the functionality of the trypsin. By adjusting the pH to 7.2 – 8 with NaOH the trypsin activity could be restored.
Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging. Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action. EDTA sequesters these ions and thus enhances the efficacy of trypsin.
Storage: -25°C to -15°C
Trypsin has a pH specification range from 7.2 to 8. At this range, phenol red inclusion gives the solution a pink color. During shipping, the trypsin might change color from pink to yellow due to exposure to carbon dioxide released from the dry ice in the shipping container. In our experience, this discoloration does not affect the functionality of the trypsin. By adjusting the pH to 7.2 – 8 with NaOH the trypsin activity could be restored.
Documents
Cell Culture Media, Molecular Buffers and Reagents
Additional Product Details
Corning Cell Culture Media Quick Reference Guide Updated.pdf Corning® Shopping list for returning to the bench VWR Corning® Media
Product Literature
Corning Cell Culture Media.pdf
Promotion Material
Product Family Options
- DescriptionSize
- : 25-051-CI
- 0.05% Trypsin 0.53 mM EDTA [-] sodium bicarbonate100 mlPack of 6 (100ml)$69.70: 25-052-CI
- 0.25%Trypsin, 2.21 mM EDTA, 1X [-] sodium bicarbonate100 mlPack of 6 (100ml)$69.60: 25-053-CI
- 0.05% Trypsin, 0.53 mM EDTA, 1X [-] sodium bicarbonate500 mlPack of 6 (500ml)$234.00: 25-052-CV
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