G-Biosciences' Avidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag

G-Biosciences' avidin is a glycoprotein with approximately 10% of its total mass coming from carbohydrates. Avidin has a molecular weight of 67 kDa and contains four identical 128 amino acid subunits that each has a single biotin binding domain. Avidin is a basic protein with an isoelectric pH of 10-10.5 and is readily soluble in aqueous buffers containing a wide range of salt, temperature and other laboratory agents. This wide range of tolerance makes avidin suitable for a variety of analytical applications. Avidin has extraordinary binding affinity for biotin (Ka=10¹⁵M¯¹). The avidin is covalently coupled to the agarose, resulting in minimal leaching, and is stable over a pH range of 2-11.

G-Biosciences' Avidin Resin is supplied as a 50% resin slurry. Specific binding and elution buffers are also offered.

Avidin Resin can also be used for immunoprecipitations using biotin labeled antibodies. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵M¯¹) and streptavidin (Ka=10¹⁵M¯¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3 M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8 M guanidine-hydrochloride at pH 1.5 or by boiling in SDS Page Sample Loading Buffer.