G-Biosciences' EZ Yeast™ Plasmid Prep kit is designed for the rapid isolation of 2µ plasmids from yeast patches or yeast grown in small liquid cultures

G-Biosciences' LongLife™ Zymolyase®, a high performance lytic enzyme preparation, efficiently releases plasmids with a yield of up to 0.1–0.3 ng for most 2™-based plasmids from a 1.5 milliliter culture (1 prep). The EZ Yeast™ Plasmid Prep kit is suitable even for low copy number yeast plasmids.

G-Biosciences' EZ Yeast™ Plasmid Prep kit can be used for plasmid isolation from colonies, patches on plate, or liquid culture. The recovered plasmid is in TE buffer and ready for use in any molecular biology application, such as E. coli transformations, PCR amplification, and other downstream applications.

The kit is adapted from the alkaline lysis of E. coli, by providing modified alkaline lysis buffers and G-Biosciences' LongLife™ Zymolyase®, a highly stable enzyme. The EZ Yeast™ Plasmid Prep kit makes it possible to isolate high yield yeast plasmid without the use of glass beads, phenol, or repeated vortexing.