About this item
G-Biosciences' Tri-Xtract™ is a convenient, ready-to-use reagent designed for the isolation of total RNA that is free from protein and DNA contamination
G-Biosciences' Tri-Xtract™ is a monophasic solution of phenol and guanidine thiocyanate that maintains the integrity of the RNA during cell disruption and homogenization, the addition of chloroform results in the separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, DNA to the interphase, and proteins to the organic phase. The total RNA is recovered by isopropyl alcohol precipitation. The DNA and proteins can also be recovered by sequential precipitation from the organic phase.
G-Biosciences' Tri-Xtract™ is suitable for the isolation of total RNA, including mRNA, hnRNA, ribosomal RNA and tRNA, from small quantities of tissues (50-100 mg) and cells (5 x 10⁶) from human, animal, plant, yeast, bacterial and viral origin. Total RNA is isolated in under an hour and the subsequent isolation of DNA and protein in less than three hours.
The isolated RNA is suitable for Northern blots, dot blot hybridization, in-vitro translation, RNase protection assays and poly (A+) selection.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Application:
- Method / Format:
- Refine target molecule:
- Sample type:
- Target molecule:
- Environmentally Preferable: