A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)
Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. Protein-S-S-Reductant™ is a ready-to-use solution that is at neutral pH and stabilized for long-term storage (1 year). Simply supplement Protein-S-S-Reductant™ in place of DTT or ß-mercaptoethanol and boil the sample, before loading your gels.
G-Biosciences also offers convenient 1 gm quantities of TCEP, bulk 5 gm quantities of DTT, and 100 ml bottles of ß-mercaptoethanol. Also available is G-Biosciences' OneQuant™ DTT, which are single aliquots of DTT that eliminate the need for weighing, preventing loss of reagent and saving time. Just add 90μl water to a single tube to generate a 0.5M DTT solution. OneQuant™ DTT kit is supplied with 40 individual tubes.
TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pH's, compared to other reductants.
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