TMB 2-Component develops a deep blue color in the presence of peroxidase-labeled conjugates measurable at 650 nm

TMB can also be used in blotting applications with the purchase of the TMB Membrane Enhancer (50-77-01). 2-component format, simply mix and use.

Turns yellow when stopped by acidification and read at 450 nm. Recommended for ELISA. Has longer shelf life than 1-component TMB substrates.