About this item
The SpinOUT™ GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of protein solutions ranging from 5µL through to 4mL sample volumes
The SpinOUT™ columns are simply to use as the protein sample is applied, and then centrifuged to recover protein, with the column retaining >95% of the salts and small molecules (<1,000Da for GT-600 and <1,500 for GT-1200). Spin-OUT™ GT-600 is for the purification of proteins > 6kDa and nucleic acids larger than 10bp. Spin-OUT™ GT-1200 is for the purification of proteins > 30kDa and removal of molecules >1,500Da. The columns are ideal for separating proteins from peptides.
The SpinOUT™ PCR columns are spin format desalting columns that have the ability to remove salts, radioisotpes, dyes, primers and deoxynucleotides (dNTPs) for nucleic acids following PCR. Two sizes are available to remove contaminating products. PCR-20 removes contaminating products from PCR products, including <20bp primers, dNTPs and salts. PCR-32 removes PCR products from <32bp primers, dNTPs and salts.
The SpinOUT™ columns are available in two MWCO sizes for >6,000 or >30,000 dalton proteins and are suitable for samples containing as little as 20µg protein/mL. Five sizes are available per each MWCO.
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