BioLegend’s ELISA MAX™ Standard Set contains the capture and detection antibodies, recombinant protein standard, and Avidin-HRP required for the accurate quantification of natural and recombinant human IFN-γ.

BioLegend’s ELISA MAX™ Standard Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A human IFN-γ specific mouse monoclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and IFN-γ binds to the immobilized capture antibody. Next, a biotinylated mouse monoclonal anti-human IFN-γ detection antibody is added, producing an antibody-antigenantibody “sandwich”. Avidin-horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IFN-γ present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.

No cross reactivity was observed when this kit was used to analyze multiple human, mouse and rat recombinant proteins.