This buffer is a concentrated antigen unmasking solution and is free from heavy metals
Fixative used on tissue sections to conserve the tissue from autolysis, preserve tissue structures, and immobilize antigens alters the tissue. Antigens undergo chemical alteration of their primary, secondary and tertiary structures. Because of changes in the protein-containing epitopes or in neighboring proteins, antigenic sites may be masked. Use of a heavy metal solution in a microwave can regain the masked antigens significantly. However, heavy metals increase the risk of exposure of lab personnel to lead. Use of this antigen unmasker can eliminate the risk of heavy metal exposure to lab personnel and also resolve heavy metals handling and disposal problems.
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