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- Description:2D-Xtract™
- Size:50 ml
- Cat. No.:95043-430
- Supplier no.:786-501
2D-Xtract™ is a dry, urea-based and ready-to-use protein solubilization buffer.
- Convenient and simple to use extraction buffer
- No preparation required, simply hydrate and use
- Prevents urea induced protein carbamylation
- Prevents waste of unused reagents
- No artifactual protein bands due to dust and human skin contamination
2D-Xtract™ is a proprietary modification of urea based chaotropic extraction buffers. Normal urea based buffers solubilize proteins effectively however can modify the native charge of the proteins, due to carbamylation. A process that modifies amino groups, inducing changes in the isoelectric point of proteinsleading to artifactual 2D results.
2D-Xtract™ is a dry, pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulfobetaine (ND SB 201) for maximumsolubilizing strength. The dry format allows researchers to freshly rehydrate as much or as little as is required and therefore prevent urea induced carbamylation.
This is suitable for sample extraction and solubilization for 2D gel electrophoresis and other applications and also suitable for rehydration of IPG Strips.