The qPCR MasterMix is supplied as a 2X product and is a ready to use HotStart qPCR MasterMix with EvaGreen® dye, FlashTaq DNA polymerase, dCTP, dGTP, dATP, dTTP, and MgCl2. EvaGreen® is a dsDNA-binding dye. EvaGreen® possesses similar absorption and emission spectra as SYBR® Green I.

• It is cell membrane impermeable
• Non-cytotoxic
• Non-mutagenic
• Safe for aquatic life

FlashTaq is chemically modified hot-start Taq DNA polymerase with an activation time of two minutes at 95 °C. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli.

Minimize number of freeze thaw cycles of frozen product.

Applications: qPCR (dye).