Human Recombinant R-Spondin-1 (from CHO cells)

R-Spondin-1 (RSPO-1) is a secreted growth factor that stabilizes β-catenin and enhances WNT signaling.RSPO-1 is expressed in many tissues and regulates female sex determination, XX gonad development, oocyte differentiation, and hematopoietic stem cell specification. The RSPO-1 stabilization of β-catenin promotes intestinal crypt cell proliferation. This suggests that RSPO-1 functions as a stem cell growth factor and may have therapeutic potential in the treatment of gastrointestinal diseases and chemotherapy-induced intestinal mucositis. Additionally, recombinant RSPO-1 protein is used to initiate and maintain intestinal organoid cultures.

High quality
Low endotoxin
Affordable

: For research use only.

Conjugation:
Unconjugated
Protein Function:
Cytokine
Protein/Peptide Type:
Recombinant
Source:
CHO cells
Species:
Human
Storage Conditions:
–20 °C
Endotoxin Content:
Endotoxin LAL (EU/µg) ≤0.1
Biological Activity:
Bioactivity Assay: Luciferase Induction in HEK-293 STF cells in the presence of Murine Wnt-3a, ED₅₀ ≤50 ng/ml, Bioactivity ≥2.0×10⁴ units/mg
Gene ID:
Q2MKA7
Reconstitution Instructions:
Sterile water at 0.1 mg/ml
Endotoxin-free:
N
Carrier-Free:
Y
Protease-free:
N
Animal-Free:
N
Protein Synonyms:
Roof plate-specific Spondin-1|hRspo1
Protein/Peptide Name:
R-Spondin-1
Purity:
≥95%
Molecular Weight:
26.8 kDa
Sequence:
SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA
Endotoxin Level:
Low
Formulation:
Phosphate buffered saline (PBS)
Nuclease-free:
N
Grade:
RUO
Shipping Temperature:
RT
Tested Applications:
Bioassay