FlashTaq™ HotStart 2X MeanGreen™ MasterMix contains FlashTaq™ HotStart DNA polymerase; a chemically modified HotStart Taq DNA polymerase.

• Application: Endpoint PCR, HotStart PCR
• Long term storage at −20 °C
• Minimise number of freeze thaw cycles of frozen product

The FlashTaq™ HotStart 2X MeanGreen™ MasterMix remains inactive at room temperature until after a twovminute activation at 95 °C. The 2X MeanGreen™ MasterMix contains dCTP, dGTP, dATP, dTTP, MgCl2, FlashTaq™ HotStart DNA Polymerase, and MeanGreen™ Dye. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli.

FlashTaq™ HotStart 2X MeanGreen™ MasterMix contains blue and yellow loading dyes and a density agent that allow reactions to be loaded directly onto agarose gels and make monitoring progress during electrophoresis easy. These dyes do not obscure visualisation of DNA bands on the gel as the dye fronts run outside the size of most PCR products.