Reverse Transcriptase System, Promega

The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in fifteen minutes.

• Convenient System for Reverse Transcription Using AMV Reverse Transcriptase

The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in 15 minutes. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and Random Primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1ug of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 separate PCR amplifications. A polyadenylated 1.2kb RNA transcript is provided as a control template for cDNA synthesis.

Components allow for up to 100 reactions.

 : The MgCl2 and reverse transcriptase buffer are both available separately.

 : The system comes with 1500 units of AMV reverse transcriptase (HC), 2500 units of recombinant RNasin ribonuclease inhibitor, 50 µg of Oligo (dT)15 primer, 50 µg of random primers, 5 µg 1.2 kb of kanamycin positive control RNA, 320 µL of dNTP mix, 1.4 mL of reverse transcription buffer (10X), 1.2 mL of MgCl2, and 13 mL of nuclease-free water.