Mouse Recombinant TNF-A (from E. coli)
Supplier: FUJIFILM IRVINE SCIENTIFIC, INC
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About this item
Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.
- High quality
- Low endotoxin
- Affordable
Specifications
- Conjugation:Unconjugated
- Protein Function:Cytokine
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Mouse
- Storage Conditions:–20 °C
- Endotoxin Content:Endotoxin LAL (EU/µg) ≤0.1
- Biological Activity:Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0.1 ng/ml, Bioactivity ≥1.0×10⁷ units/mg
- Gene ID:P06804
- Amino Acid Number:157
- Reconstitution Instructions:Sterile water at 0.1 mg/ml
- Endotoxin-free:N
- Carrier-Free:Y
- Protease-free:N
- Animal-Free:Y
- Protein Synonyms:Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
- Protein/Peptide Name:TNF-A (AF)
- Purity:≥95%
- Molecular Weight:17.4 kDa
- Sequence:MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
- Endotoxin Level:Low
- Formulation:10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
- Nuclease-free:N
- Grade:RUO
- Shipping Temperature:RT
- Tested Applications:Bioassay