Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Rat
- Target protein:OxLDL
- Size:1 Kit
- Sample Type:Serum, plasma and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Oxidized Low Density Lipoprotein (OxLDL) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:31.2 - 2000 pg/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:13.9 pg/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEA527RA
- No. of tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting Rat OxLDL (Oxidized Low Density Lipoprotein). The assay range is from 31.2 to 2000 pg/ml (Sandwich kit) with a sensitivity of 13.9 pg/ml. There is no detectable cross to reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-Assay: CV <10%, Inter-Assay: CV <12%
Hypercholesterolemia is closely associated with increased risk of atherosclerosis. The cholesterol accumulated in the atherosclerotic plaque is derived primarily from low-density lipoprotein (LDL). Oxidation of LDL is now accepted as a critical event in the atherogenic process. Free radicals like superoxide and nitric oxide (−O₂ , NO) generated in biological reactions in the body contribute to the oxidation of LDL. The NO radical has been shown to oxidize apolipoprotein B a constituant of LDL. Similarly, lipoxygenases and oxidants like peroxynitrite can oxidize the lipid moieties in LDL. It has been demonstrated that oxLDL and not native LDL is taken up by scavenger receptors on monocytes, smooth muscle cells and macrophages in the blood vessels. The oxidation of LDL increases the affinity of oxLDL to acetyl receptors of macrophages. As this pathway of oxLDL uptake is unregulated, it results in the formation of lipid-laden macrophages (foam cells).