Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Rat
- Target protein:MIF
- Size:1 Kit
- Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Macrophage Migration Inhibitory Factor (MIF) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:31.2 - 2000 pg/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:12.2 pg/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEA698RA
- No. of tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting Rat MIF (Macrophage Migration Inhibitory Factor). The assay range is from 31.2 to 2000 pg/ml (Sandwich kit) with a sensitivity of 12.2 pg/ml. There is no detectable cross to reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-Assay: CV <10%, Inter-Assay: CV <12%
One of the first cytokines described, MIF (macrophage migration inhibitory factor) was originally identified in studies of delayed hypersensitivity reactions where it was shown to inhibit macrophage migration. It is an important mediator of the innate immune response with potential roles in the pathophysiology of inflammatory, autoimmune, and neoplastic disorders. The human MIF gene encodes a 115 amino acid, 12.5 kDa secreted protein. Crystallographic studies suggest that MIF exists as a homotrimer, although some reports show that it may exist as a dimer or monomer as well. Although MIF exhibits no homology with other known cytokines, it shares structural homology with several bacterial enzymes. It has been speculated that MIF is an inflammatory mediator possibly associated with rheumatoid arthritis (RA) severity.