Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Rat
- Target protein:HMGB1
- Size:1 Kit
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMGB1) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:31.2 - 2000 pg/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:12.2 pg/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEA399RA
- No. of tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting Rat HMGB1 (High Mobility Group Protein 1). The assay range is from 31.2 to 2000 pg/ml (Sandwich kit) with a sensitivity of 12.2 pg/ml. There is no detectable cross to reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-Assay: CV <10%, Inter-Assay: CV <12%
High mobility group box 1 is a non-histone chomosomal protein that functions in endotoxin lethality. It is released from activated macrophages and is present at elevated levels in the serum of sepsis patients. HMGB1 was also reported to interact with RAGE (receptor for advanced glycation end products) and to influence neuron development.A large number of sequences related to the cDNA for HMG1 protein hampered the cloning and mapping of the active HMG1 gene. Ferrari et al., (1996) showed that the HMG1 gene contains introns, whereas the HMG1-related sequences do not and most likely are retrotransposed pseudogenes. They identified 8 YACs from the ICI and CEPH libraries that contain the human HMG1 gene. The gene is similar in structure to the previously characterized mouse homolog.