Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Rat
- Target protein:COMP
- Size:1 Kit
- Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Cartilage Oligomeric Matrix Protein (COMP) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:6.25 - 400 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:3.02 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEB197RA
- No. of tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting Rat COMP (Cartilage Oligomeric Matrix Protein). The assay range is from 6.25 to 400 ng/ml (Sandwich kit) with a sensitivity of 3.02 ng/ml. There is no detectable cross to reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-Assay: CV <10%, Inter-Assay: CV <12%
Cartilage oligomeric matrix protein (COMP) is a member of the thrombospondin family of extracellular matrix proteins. The protein consists of five 87-kDa subunits held together by interchain disulfide bonds, forming a 435-kDa pentameric protein. COMP is expressed in all types of cartilage, vitreous of the eye, tendon, and vascular smooth muscle cells. Immunohistological staining of articular cartilage has revealed a developmentally regulated localization of COMP to the chondrocyte territorial and interterritorial matrix. COMP binds in a zinc-dependent manner to collagen type I and type II and also to collagen type IX. The thrombospondin family currently includes five members: TSP-1, TSP-2, TSP-3, TSP-4, and COMP (also referred to as TSP-5).