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Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:HumanRatMouse
- Target protein:SMAD3
- Size:1 Kit
- Sample Type:Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between SMAD family member 3 (SMAD3) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:0.625 - 40 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:0.233 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEC123MI
- No. of tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting Human/Mouse/Rat SMAD3 (SMAD family member 3). The assay range is from 0.625 to 40 ng/ml (Sandwich kit) with a sensitivity of 0.233 ng/ml. There is no detectable cross to reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-Assay: CV <10%, Inter-Assay: CV <12%
SMAD3 or Mothers against decapentaplegic homolog 3 is a gene which appears in humans on chromosome 15. It is one of several human homologues of a gene which was originally discovered in the fruit fly Drosophila melanogaster. The name SMAD3 also refers to a member of the SMAD family of proteins. SMAD3 or Mothers against decapentaplegic homolog 3 is a polypeptide that, as its name describes, is a homolog of the Drosophila protein 'Mothers against decapentaplegic'. It belongs to the SMAD family of proteins, which belong to the TGF beta superfamily of modulators. Like many other TGF beta family members, SMAD3 is involved in cell signalling. SMAD3 modulates signals of activin and TGF beta's.