This assay has high sensitivity and excellent specificity for detecting General species 5 to Me to THF (5 to Methyltetrahydrofolate). The assay range is from 1.11 to 90 ng/ml (Competitive kit) with a sensitivity of 0.43 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

• High sensitivity and specificity
• Perfect reproducibility and consistency across batches
• Quality control with three-level inspections
• Wide range of targets/species available
• Intra-Assay: CV <10%, Inter-Assay: CV <12%

5-Methyltetrahydrofolate, the predominant metabolic form of folate, is a methylated derivate of tetrahydrofolate. It is generated by methylenetetrahydrofolate reductase (MTHFR) from 5, 10-methylenetetrahydrofolate and used to recycle homocysteine back to methionine by 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) or methionine synthase (MS). Levomefolic acid (INN) or metafolin is the natural, active form of folic acid used at the cellular level for DNA reproduction, the cysteine cycle and the regulation of homocysteine among other functions. The un-methylated form, folic acid (vitamin B9), is a synthetic form of folate found in nutritional supplements. Synthetic folic acid is metabolized in the body into levomefolic acid. Approximately 10% of the general population (homozygous TT) lack the enzymes needed to receive any benefit from folic acid.