Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Rat
- Target protein:FGF15
- Size:1 Kit
- Sample Type:Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Fibroblast Growth Factor 15 (FGF15) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:2 h
- Shelf Life:12 Months
- Detection Range:12.5 - 200 pg/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:50 µl
- Sensitivity:5.1 pg/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-CEL154RA
- No. of tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting Rat FGF15 (Fibroblast Growth Factor 15). The assay range is from 12.5 to 200 pg/ml (Competitive kit) with a sensitivity of 5.1 pg/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-Assay: CV <10%, Inter-Assay: CV <12%
FGF15 is the mouse ortholog of human FGF19 (hence there is no human FGF15). Fibroblast growth factors, or FGFs, are a family of growth factors involved in angiogenesis, wound healing, and embryonic development. The FGFs are heparin-binding proteins and interactions with cell-surface associated heparan sulfate proteoglycans have been shown to be essential for FGF signal transduction. FGFs are key players in the processes of proliferation and differentiation of wide variety of cells and tissues. Fibroblast growth factor was found in pituitary extracts by Armelin in 1973 and then was also found in a cow brain extract by Gospodarowicz et al., and tested in a bioassay which caused fibroblasts to proliferate (first published report in 1974).