- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Mouse
- Target protein:PINP
- Size:1 Kit
- Sample Type:Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Procollagen I N-Terminal Propeptide (PINP) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:2 h
- Shelf Life:12 Months
- Detection Range:370.4 - 30000 pg/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:50 µl
- Sensitivity:138.2 pg/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-CEA957MU
- No. of tests:96 wells
This assay has high sensitivity and excellent specificity for detecting Mouse PINP (Procollagen I N to Terminal Propeptide). The assay range is from 370.4 to 30,000 pg/ml (Competitive kit) with a sensitivity of 138.2 pg/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-Assay: CV <10%, Inter-Assay: CV <12%
The PINP molecule is similar to PIIINP consisting of three distinct structural domains: Col 1 is on the aminoterminal side of the molecule, while Col 2 and Col 3 are situated on the middle of the helically structured molecule (Kühn et al., 1982). The PINP molecule has a molecular mass of 35000 and is cleared by scavenger receptors in liver endothelial cells (Melkko et al., 1994). PINP often occurs in circulation in two forms of different molecular sizes. One is identical to the trimeric authentic antigen (intact PINP) whereas the other consists of smaller forms of PINP, resembling a single domain of the proα1 (I) chain of PINP and is probably a degradation product of type I procollagen or I pN-collagen. Thus, an assay of intact PINP rather than total PINP appears to be more sensitive in detecting changes in the rate of type I collagen synthesis (Melkko et al., 1996, Risteli & Risteli 1999).