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Mouse COL4 ELISA Kit
  MSPP-SEA180MU
 :  
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Mouse COL4 ELISA Kit
  MSPP-SEA180MU
 :  SEA180MU
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Sandwich
  • Conjugate ELISA:
    Biotin
  • Format:
    Pre-coated
  • Host:
    Rabbit
  • Primary antibody reactivity:
    Mouse
  • Target protein:
    COL4
  • Size:
    1 kit
  • Sample Type:
    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Cross Reactivity:
    No significant cross-reactivity or interference between Collagen Type IV (COL4) and analogues was observed
  • Detection Method:
    Colorimetric
  • Time to Results:
    3 h
  • Shelf Life:
    12 Months
  • Detection Range:
    0.156 - 10 ng/ml
  • Storage Temperature:
    4 °C for one month (frequent use), −20 °C for one year
  • Sample Volume:
    100 µl
  • Sensitivity:
    0.062 ng/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    MSPP-SEA180MU
  • No. of tests:
    96 wells

 

 

This assay has high sensitivity and excellent specificity for detecting Mouse COL4 (Collagen Type IV). The assay range is from 0.156 to 10 ng/ml (Sandwich kit) with a sensitivity of 0.062 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

  • High sensitivity and specificity
  • Perfect reproducibility and consistency across batches
  • Quality control with three-level inspections
  • Wide range of targets/species available
  • Intra-assay: CV<10%; Inter-assay: CV<12%

Type-IV collagen is a type of collagen found primarily in the basal lamina. The C-terminus domain is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, type-IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina. All of the type IV collagen in mammals is derived from six genetically distinct a-chain polypeptides (a1–a6). The type IV collagen a-chains have similar domain structures and share between 50–70% homology at the amino-acid level. The a-chains can be separated into three domains: an amino-terminal 7S domain, a middle triple-helical domain, and a carboxy-terminal globular non-collagenous (NC)-1 domain (see figure).

 : For research use only. Not for use in clinical diagnostic procedures. Please proper stored each component based on the instruction.