This assay has high sensitivity and excellent specificity for detecting Mouse ABP1 (Amiloride Binding Protein 1). The assay range is from 31.2 to 2000 pg/ml (Sandwich kit) with a sensitivity of 13.2 pg/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

• High sensitivity and specificity
• Perfect reproducibility and consistency across batches
• Quality control with three-level inspections
• Wide range of targets/species available
• Intra-assay: CV<10%; Inter-assay: CV<12%

On the basis of its primary structure, the amiloride-binding protein (EC 1.4.3.6) is 713 amino acids long, with a 19-amino acid signal peptide. Expressed in cultured cells, the mRNA yields a glycoprotein that binds amiloride and amiloride analogs with affinities similar to the amiloride receptor associated with the apical Na⁺ channel in pig kidney membranes and is immunoprecipitated with monoclonal antibodies raised against pig kidney amiloride-binding protein. Barbry et al., (1990) pointed out that amiloride-sensitive Na⁺ channels are also present in airway epithelia, where they play an important role in fluid secretion. Amiloride inhibits the excessive absorption of Na⁺ and liquid that takes place in airway epithelia of patients with cystic fibrosis, and amiloride aerosol therapy has been tried for the treatment of lung disease in CF.