Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Human
- Target protein:Tie1
- Size:1 kit
- Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:1.56 - 100 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:0.62 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEA125HU
- No. of tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting Human Tie1 (Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1). The assay range is from 1.56 to 100 ng/ml (Sandwich kit) with a sensitivity of 0.62 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
Tie1 is a cell surface protein expressed exclusively in endothelial cells. Tie1 upregulates the cell adhesion molecules VCAM-1, E-selectin, and ICAM-1 through a p38-dependent mechanism. Attachment of monocyte derived immune cells to endothelial cells is also enhanced by Tie1 expression. Tie1 has a proinflammatory effect and may play a role in the endothelial inflammatory diseases such as atherosclerosis. the combinatorial function of angiopoietin-1 and the orphan receptor TIE1 is critical for the development of the right-hand side venous system but is dispensable for the left-hand side venous system. This finding revealed the existence of a distinct genetic program for the establishment of the right-hand side and left-hand side vascular networks well before the network asymmetry becomes morphologically discernible.