- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Human
- Target protein:S100A12
- Size:1 kit
- Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between S100 Calcium Binding Protein A12 (S100A12) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:78 - 5000 pg/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:33 pg/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEB080HU
- No. of tests:96 wells
This assay has high sensitivity and excellent specificity for detecting Human S100A12 (S100 Calcium Binding Protein A12). The assay range is from 78 to 5000 pg/ml (Sandwich kit) with a sensitivity of 33 pg/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
S100A12 belongs to the S-100 (soluble in 100% saturated ammonium sulfate) family. Similar to other S100 proteins, S100A12 is small, it only has 91 amino acids, contains 2 EF-hand calcium-binding motifs and a Hinge domain. The hinge domain contributes significantly to its chemotactic properties. In the absence of divalent cations, S100A12 forms noncovalent homodimers; in the presence of calcium, zinc and copper ions, it forms tetramers or hexamers. S100A12 can interact with AGER and both calcium and zinc are essential for the interaction; it can Interact with CACYBP in a calcium-dependent manner. Besides, it can form heterodimers with S100A9. S100A12 plays a prominent role in the regulation of inflammatory processes and immune response. The concentration of S100A12 is high in the serum of patients suffering from various inflammatory disorders, including rheumatoid arthritis, psoriatic arthritis, Crohn's disease, ulcerative colitis, and Kawasaki disease.