Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Human
- Target protein:RNASE7
- Size:1 kit
- Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Ribonuclease A7 (RNASE7) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:1.56 - 100 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:0.62 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SED193HU
- No. of tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting Human RNASE7 (Ribonuclease A7). The assay range is from 1.56 to 100 ng/ml (Sandwich kit) with a sensitivity of 0.62 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
RNASE7 is a member of the RNase A superfamily that has broad-spectrum antimicrobial activity and ribonuclease activity. Harder and Schroder showed that recombinant RNASE7 displayed catalytic activity against yeast tRNA. They found that RNASE7 exhibited broad-spectrum antimicrobial activity against several gram-positive and gram-negative bacteria, and against Candida albicans, as well as vancomycin-resistant E. faecium. RNASE7 expression was induced upon treatment with interferon gamma, interleukin-1-beta, and to a lesser extent by TNF-alpha. Zhang et al., also identified broad spectrum RNASE7 antimicrobial activity. However, they determined that RNASE7 did not display antiviral activity against respiratory syncytial virus infectivity for human epithelium cells using an in vitro assay. They confirmed that recombinant RNASE7 displayed catalytic activity against yeast tRNA.