- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Human
- Target protein:DCD
- Size:1 kit
- Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Dermcidin (DCD) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:0.78 - 50 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:0.29 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEC896HU
- No. of tests:96 wells
This assay has high sensitivity and excellent specificity for detecting Human DCD (Dermcidin). The assay range is from 0.78 to 50 ng/ml (Sandwich kit) with a sensitivity of 0.29 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
Dermcidin is a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients. High performance liquid chromatography, mass spectrometry, and microsequence analysis of sweat showed a processed 47-amino acid DCD peptide with a calculated mass of 4.7 kD, compared with the expected 9.3 kD of full-length DCD without the signal peptide.DCD expression in skin, melanocytic nevus, and cutaneous melanoma tissue, but not in any other organ systems tested.