Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Human
- Target protein:COMP
- Size:1 kit
- Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Cartilage Oligomeric Matrix Protein (COMP) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:3.12 - 200 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:1.21 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEB197HU
- No. of tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting Human COMP (Cartilage Oligomeric Matrix Protein). The assay range is from 3.12 to 200 ng/ml (Sandwich kit) with a sensitivity of 1.21 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
Cartilage oligomeric matrix protein (COMP) is a member of the thrombospondin family of extracellular matrix proteins. The protein consists of five 87-kDa subunits held together by interchain disulfide bonds, forming a 435-kDa pentameric protein. COMP is expressed in all types of cartilage, vitreous of the eye, tendon, and vascular smooth muscle cells. Immunohistological staining of articular cartilage has revealed a developmentally regulated localization of COMP to the chondrocyte territorial and interterritorial matrix. COMP binds in a zinc-dependent manner to collagen type I and type II and also to collagen type IX. The thrombospondin family currently includes five members: TSP-1, TSP-2, TSP-3, TSP-4, and COMP (also referred to as TSP-5).