- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Human
- Target protein:CLDN3
- Size:1 kit
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Claudin 3 (CLDN3) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:0.312 - 20 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:0.112 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEF293HU
- No. of tests:96 wells
This assay has high sensitivity and excellent specificity for detecting Human CLDN3 (Claudin 3). The assay range is from 0.312 to 20 ng/ml (Sandwich kit) with a sensitivity of 0.112 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are composed of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. Claudin 3 encoded by this intron-less gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares amino acid sequence similarity with a putative apoptosis-related protein found in rat.