- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Human
- Target protein:APOM
- Size:1 kit
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Apolipoprotein M (APOM) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:1.56 - 100 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:0.64 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEC299HU
- No. of tests:96 wells
This assay has high sensitivity and excellent specificity for detecting Human APOM (Apolipoprotein M). The assay range is from 1.56 to 100 ng/ml (Sandwich kit) with a sensitivity of 0.64 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
Apolipoprotein M is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized.The deduced 188-amino acid protein has a calculated molecular mass of about 21 kD. It contains a potential N-glycosylation site, two possible disulfide bridges, and a hydrophobic alpha-helical signal peptide that is retained in the mature protein. Sequence analysis revealed 79% identity between the human and mouse proteins.