Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Multiple
- Target protein:KNM
- Size:1 kit
- Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Kanamycin (KNM) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:2 h
- Shelf Life:12 Months
- Detection Range:617.3 - 50000 pg/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:50 µl
- Sensitivity:253.4 pg/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-CEK966GE
- No. of tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting General species KNM (Kanamycin). The assay range is from 617.3 to 50000 pg/ml (Competitive kit) with a sensitivity of 253.4 pg/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
Kanamycin is an aminoglycoside bacteriocidal antibiotic, available in oral, intravenous, and intramuscular forms, and used to treat a wide variety of infections. Kanamycin is isolated from the bacterium Streptomyces kanamyceticus and its most commonly used form is kanamycin sulfate. Kanamycin interacts with the 30S subunit of prokaryotic ribosomes. It induces substantial amounts of mistranslation and indirectly inhibits translocation during protein synthesis. Kanamycin is used in molecular biology as a selective agent most commonly to isolate bacteria which have taken up genescoupled to a gene coding for kanamycin resistance. Bacteria that have been transformed with a plasmid containing the kanamycin resistance gene are plated on kanamycin (50 to 100 µg/ml) containing agar plates or are grown in media containing kanamycin (50 to 100 µg/ml).