Specifications
- Pack type:Vial
- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Multiple
- Size:50 µg
- Tag sequence:MGHHHHHHSGSEF
- Storage Conditions:–20 °C
- Endotoxin Content:<1.0 EU per 1 μg (determined by the LAL method)
- Reconstitution Instructions:Reconstitute in 10 mM PBS (pH 7.4) to a concentration of 0.1 - 1.0 mg/ml. Do not vortex.
- Endotoxin-free:N
- Carrier-Free:Y
- Protease-free:N
- Animal-Free:Y
- Protein Synonyms:Glutathione S Transferase
- UniProtKB:P08515
- Protein/Peptide Name:GST
- Purity:95 - 100%
- Molecular Weight:26 kDa
- Sequence:Met1~Lys218
- Endotoxin Level:Low
- Concentration:0.2 mg/ml
- Formulation:Lyophilized from PBS, pH 7.4 containing 0.01% SKL, 5% trehalose
- Nuclease-free:N
- Shipping Temperature:4 °C
- Tested Applications:Positive control, Immunogen, SDS-PAGE, Western blot.
- Cat. No.:MSPP-RPX158GE1
Specifications
About this item
This is a GST recombinant protein (prokaryotic), General species is sequencing from Met1~Lys218 with 95 to 100% purity. LyopH ilized from PBS, pH 7.4, containing 0.01% SKL, 5% Trehalose with 0.2 mg/ml.
- High quality, purity, reproducibility and effectiveness
- Offers customized buffers and tag options
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Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal also known as MAPEG-proteins.
A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids(roughly 26 kDa) in size, which compared to tags such as theMyc-tagor theFLAG-tag is quite large. It can be fused to either theN-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include athrombindomain for cleavage of the GST tag during protein purification.