Assay-specific Crystal Flex Probes and primers for the detection of PIK3CA mutations and its positive control. PIK3CA (Ref, H1047L, E542K, E545K, H1047R, N345K) Crystal Digital PCR® Assay is a 10X assay designed to detect and quantify 5 mutations in the PIK3CA gene using the Ruby Chip. PIK3CA is essential for regulating multiple cellular processes through the PI3K/AKT/mTOR signaling pathway including cell growth, proliferation, survival, and metabolism.

• Ready-to-use assay reagents to detect multiple targets in a single reaction
• Crystal Flex Probe detection chemistry for flexible assay configuration
• Same set of crystal universal Reporters to detect any target in different assays
• High multiplexing with low price per point

Wet lab tested
Compatibility: Nio™ Digital PCR, Prism6, ruby chip
Channels: Red, blue, teal, green, yellow, infra-red
Required reagents: Crystal universal reporter 7 and naica® PCR MIX 10X
Target: PIK3CA Ref/H1047L/E542K/E545K/H1047R/N345K