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- Pack type:1 Vial
- Cat. No.:TCR0238-1VIAL
The rSRL is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. This lectin preferably binds to [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] glycan epitopes by its two distinct carbohydrate-binding sites.
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rSRL-LecBeads (1 ml gel) shows a binding capacity of about 7.5 mg or more agalacto human Transferrin [agalacto hTF] through its binding to a terminal [GlcNAcβ(1-2)Man] glycan epitope on agalacto hTF.
- Preparation : Recombinant SRL is covalently coupled with agarose gel
- Quantity : 1 ml gel/2 ml (1:1, v/v slurry) suspended in PBS, pH7.5
- Protein concentration : about 15 mg/ml gel
- Binding capacity : >7.5 mg of glycoprotein (agalacto human Transferrin) /ml gel
- Preservative : 0.02% [w/v] Sodium azide
- Stabilizer : None
- Store at 2-10 °C. (Avoid freezing).