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rPSL1a-LecBeads [for Siaα(2-6)Gal]
  TCR0235-1VIAL
New Product
 :  
rPSL1a-LecBeads [for Siaα(2-6)Gal]
  TCR0235-1VIAL
 :  R0235-1VIAL
New Product
 :  

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  • Pack type:
    1 Vial
  • Cat. No.:
    TCR0235-1VIAL

 

 

The rPSL1a is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Polyporus squamosus. This lectin preferably binds to a [Siaα(2-6)Gal] glycan epitope (It very slightly interacts with lactose.).
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rPSL1a-LecBeads (1 ml gel) shows a binding capacity of about 10 mg or more human Transferrin [hTF] through its binding to a terminal [Siaα(2-6)Gal] glycan epitope on hTF.

  • Preparation : Recombinant PSL1a is covalently coupled with agarose gel
  • Quantity : 1 ml gel/2 ml (1:1, v/v slurry) suspended in PBS, pH7.5
  • Protein concentration : about 10 mg/ml gel
  • Binding capacity : >10 mg of glycoprotein (human Transferrin) /ml gel
  • Preservative : 0.02% [w/v] Sodium azide
  • Stabilizer : None
  • Store at 2-10 °C. (Avoid freezing).