Assay-specific Crystal Flex Probes and primers for the detection of TERT mutations and its Positive Control. TERT (Ref/SLC12A7/228T/C242T-C243T/C250T/TSN) Crystal Digital PCR® Assay is a 10X assay designed to detect and quantify 3 mutations in the TERT gene using the Ruby Chip. Three references are detected and used to quantify TERT gene amplification: a reference on the TERT gene, a reference on a gene located on the same chromosome as TERT (SLC12A7) and a reference on a different chromosome (TSN).

• Ready-to-use assay reagents to detect multiple targets in a single reaction
• Crystal Flex Probe detection chemistry for flexible assay configuration
• Same set of crystal universal reporters to detect any target in different assays

Wet lab tested
Compatibility: Nio™ Digital PCR, Prism6, ruby chip
Channels: Blue, green, yellow, red, infra-red, teal
Required reagents: Crystal universal reporter 7 and naica® PCR MIX 10X
Target: TERT Ref/C228T/C242T-C243T/C250T, SLC12A7, TSN

TERT plays a critical role in cellular mechanisms linked to cancer progression, notably in maintaining telomere length and promoting immortalization.
Base changes:
TERT C228T: c.-124C >T
TERT C242T-C243T: c.-138/c.-139 C >T
TERT C250T: c.-146C >T