Assay-specific Crystal Flex Probes and primers for the detection of BRAF mutations and its Positive Control. BRAF (V600E, drop-off V600) Crystal Digital PCR® Assay is a 10X assay designed to detect and quantify mutations in codons V600-K601 plus 1 mutation in the BRAF gene. BRAF is pivotal in regulating cell signaling pathways implicated in cancer development, notably melanoma, colorectal cancer and thyroid cancer, through its role in the MAPK/ERK pathway. Mutations in BRAF, particularly the V600E mutation, lead to uncontrolled cell proliferation and survival.

• Ready-to-use assay reagents to detect multiple targets in a single reaction
• Crystal Flex Probe detection chemistry for flexible assay configuration
• Same set of crystal universal reporters to detect any target in different assays

Wet lab tested
Compatibility: Nio™ Digital PCR, Prism6, Prism3, ruby chip
Channels: Red, blue, green
Required reagents: Crystal universal reporter 3 and naica® PCR MIX 10X
Target: BRAF V600-K601/V600E