Human Native ACTB (from Phlatelets)

Specifications

• Pack type:
  Vial
• Size:
  25 µg
• Storage Conditions:
  4 °C
• Molecular Weight:
  Non-muscle actin has an approximate molecular weight of 43 kDa
• Protease-free:
  No
• Reconstitution Instructions:
  The protein should be reconstituted to 10 mg/ml with 100 µl of distilled water, it will then be in the following buffer: 5 mM Tris-HCl pH 8.0, 0.2 mM CaCl₂, 0.2 mM ATP, 5% (w/v) sucrose, and 1% (w/v) dextran.
• Endotoxin Level:
  Low
• Animal-Free:
  No
• UniProtKB:
  P60709
• Purity:
  Protein purity is determined by scanning densitometry of Coomassie blue stained protein on a 12% polyacrylamide gel. Non-muscle actin was found to be >99% pure.
• Tested Applications:
  Identification and characterization of non-muscle actin binding proteins,<i>in vitro</i> actin polymerization studies,antibody standard for western blot analysis
• Carrier-Free:
  No
• Grade:
  Ultrapure grade
• Source:
  Phlatelets
• Nuclease-free:
  No
• Cat. No.:
  77538-546
• Shipping Temperature:
  Blue ice
• Biological Activity:
  The biological activity of non-muscle actin can be determined by its ability to efficiently polymerize into filaments in vitro and separate from unpolymerized components in a spin down assay. Stringent quality control ensures that >85% of the non-muscle actin can polymerized in this assay.
• Protein/Peptide Type:
  Native
• Protein Synonyms:
  actin, beta
• Protein/Peptide Name:
  ACTB
• Conjugation:
  Unconjugated
• Endotoxin-free:
  No
• Species:
  Human