Specifications
- Application:
- Description:DepleteX® RNA depletion (globin)
- Size:24 reactions
- Environmentally Preferable:
- Cat. No.:77575-226
Specifications
About this item
The DepleteX® globin depletion kit selectively removes highly expressed globin transcripts (HBA1, HBA2, HBB, and HBD) in vitro before sequencing.
- Unbiased removal of human globin from RNA-seq libraries
- Achieve >99% depletion of contaminating globin
- Rapid one-hour depletion incubation
Enhance RNA-Seq quality by eliminating globin fragments. Apart from ribodepletion, RNA samples often contain a limited number of well-characterized, highly expressed transcripts. However, resequencing these transcripts contributes minimally to biologically relevant information. The dominance of highly expressed transcripts hinders coverage for low-expressing counterparts, resulting in reduced sensitivity for detecting disease-related low-expressing genes, rare variants, and unique fusion events.
Globin mRNA dominates the mRNA content of red blood cells and whole blood. Three globlin genes (HBA1, HBA2 and HBB) constitute ≥30% of total mRNA from blood.