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DepleteX® RNA Depletion (Globin)
DepleteX® RNA Depletion (Globin)
Catalog # 77575-226
New Product
CAS Number:  
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DepleteX® RNA Depletion (Globin)
Catalog # 77575-226
Supplier Number:  NOVA-512870
New Product
CAS Number:  

Specifications

  • Application:
  • Description:
    DepleteX® RNA depletion (globin)
  • Size:
    24 reactions
  • Environmentally Preferable:
  • Cat. No.:
    77575-226

Specifications

About this item

The DepleteX® globin depletion kit selectively removes highly expressed globin transcripts (HBA1, HBA2, HBB, and HBD) in vitro before sequencing.

  • Unbiased removal of human globin from RNA-seq libraries
  • Achieve >99% depletion of contaminating globin
  • Rapid one-hour depletion incubation

Enhance RNA-Seq quality by eliminating globin fragments. Apart from ribodepletion, RNA samples often contain a limited number of well-characterized, highly expressed transcripts. However, resequencing these transcripts contributes minimally to biologically relevant information. The dominance of highly expressed transcripts hinders coverage for low-expressing counterparts, resulting in reduced sensitivity for detecting disease-related low-expressing genes, rare variants, and unique fusion events.

Globin mRNA dominates the mRNA content of red blood cells and whole blood. Three globlin genes (HBA1, HBA2 and HBB) constitute ≥30% of total mRNA from blood.

Certifications: Manufactured in ISO 9001 facility.
Caution: For research use only. Not for use in diagnostic procedures.