The DepleteX® high expressing RNA depletion kit selectively removes the most highly expressed transcripts in vitro before sequencing.

• Efficiently remove >4,000 highly expressed transcripts from whole blood and fibroblasts
• Allocate approximately 70% more reads to biologically relevant low-expressing transcripts
• Enhance variant and isoform detection while minimizing sequencing requirements
• Improved detection for low-expressing transcripts
• Seamlessly integrate into your workflow using a straightforward and adaptable protocol

Improve detection sensitivity in fibroblasts and whole blood. While resequencing highly expressed transcripts contributes minimally to biologically relevant information, these transcripts dominate sequencing coverage, hindering detection of low-expressing genes, rare variants, and unique fusion events associated with diseases. DepleteX® utilizes Cas9 and a guide RNA set to selectively remove 70% of the most highly expressed transcripts from poly-A selected RNA-Seq libraries obtained from fibroblasts and whole blood. This design optimizes the reallocation of sequencing reads, enhancing coverage of rare, low-expressing transcripts and unique fusion events.

The DepleteX® high expressing RNA depletion kit is used during the RNA-seq library prep workflow starting with fibroblasts and whole blood samples after the ligation step and before the PCR step. This kit is compatible with most RNA-seq workflows; however, it is recommended using it with the NEXTFLEX® Rapid Directional RNA-seq kit 2.0.