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- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Conjugate ELISA:Peroxidase
- Format:Pre-coated
- Host:Goat
- Primary antibody reactivity:Human
- Target protein:Metanephrine
- Description:Metanephrine (Plasma) ELISA
- Size:1×96 wells
- Sample Type:EDTA plasma, Heparin plasma
- Detection Method:Colorimetric
- Detection Range:15.1 - 3600 pg/ml
- Sensitivity:14.9 pg/ml
- Regulatory Status:RUO
- Cat. No.:101720-950
- Supplier no.:17METHUE01FST
The metanephrine ELISA is a competitive immunoassay for the quantitative determination of free metanephrines in EDTA and heparin plasma samples. The antibodies used in this test kit only recognize the biologically relevant L-forms of metanephrines.
Metanephrine (metadrenaline) is first extracted using an ion exchange matrix followed by an acylation process. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls, and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgGperoxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.