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Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Conjugate ELISA:Peroxidase
- Format:Pre-coated
- Host:Goat
- Primary antibody reactivity:Human
- Target protein:Epinephrine and norepinephrine
- Description:Bi-CAT (epinephrine and norepinephrine) ELISA
- Size:2×96 wells
- Sample Type:Plasma, urine
- Detection Method:Colorimetric
- Time to Results:4 h
- Regulatory Status:RUO
- Cat. No.:101721-322
- Supplier no.:17-BCTHU-E02.1
Specifications
About this item
The Bi-CAT ELISA (Epinephrine and Norepinephrine ELISA) is an enzyme Immunoassay for the quantitative determination of adrenaline (epinephrine) and noradrenaline (norepinephrine) in plasma and urine.
Adrenaline (epinephrine) and noradrenaline (norepinephrine) are extracted by using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.