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- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:Insulin
- Description:Mouse ultrasensitive insulin ELISA jumbo pack (10-plates)
- Size:10×96 wells
- Sample Type:Plasma, serum
- Detection Method:Colorimetric
- Time to Results:2 h, 30 min
- Detection Range:0.18 - 6.9 ng/ml or 0.025 - 1.25 ng/ml (see insert)
- Sample Volume:5 or 25 µl (hypoinsulinemic)
- Sensitivity:0.115 ng/ml or 0.019 ng/ml (see insert)
- Regulatory Status:RUO
- Cat. No.:101721-594
- Supplier no.:80-INSMSU-E10
The mouse ultrasensitive insulin jumbo (10-plate) ELISA quantifies the concentration of insulin protein products in mouse I and mouse II proinsulin genes from as little as 5 µl of serum or plasma sample.
- Only 5 µl of sample required for tail snips
- Dynamic range of 0.18 to 6.9 ng/ml
- Superior sensitivity of 0.115 ng/ml
- Only 25 µl of sample required for hypoinsulinemic samples
- Dynamic range of 0.025 to 1.25 ng/ml
- Superior sensitivity of 0.019 ng/ml
The mouse ultrasensitive insulin ELISA jumbo pack format quantifies the concentration of the insulin protein products of the mouse I and mouse II proinsulin genes from as little as 5 µl of serum or plasma sample. Utilizing a dual-monoclonal antibody sandwich ELISA format, bi-level control set, and 96-well microplate comprising removable strips, the ELISA has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components and the resealable microplate pouch allow for convenient storage for future use if an entire plate is not needed at one time.
The mouse ultrasensitive insulin ELISA jumbo has seven standards designed to meet the needs of either very small sample size or very low insulin concentrations. For small samples, such as tail snip samples, use a sample size of 5 µL and the top five standards, 0.18 to 6.9 ng/ml. For hypoinsulinemic samples from a type 1 diabetes model or streptozotocin treatment, use a 25 µl sample size and the bottom five standards, 0.025 to 1.25 ng/ml.