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Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Mouse
- Target protein:C-peptide
- Description:Mouse C-peptide ELISA
- Size:1×96 tests
- Environmentally Preferable:
- Sample Type:serum
- Detection Method:Colorimetric
- Time to Results:2 h, 15 min
- Detection Range:60 - 3000 pM
- Sensitivity:7.6 pM
- Regulatory Status:RUO
- Cat. No.:101720-804
- Supplier no.:80-CPTMS-E01
Specifications
About this item
The Mouse C-peptide ELISA quantifies the C-peptide protein products of mouse I and mouse II proinsulin genes. The assay utilizes a dual-monoclonal antibody sandwich format, bi-level control set, and 96-well microplate comprising removable strips. A single Mouse C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. Only 10 µl of sample is sufficient for the assay.
- Superior analytical sensitivity of 7.6 pmol/L
- Broad dynamic range of 60 to 3000 pmol/L
The ALPCO mouse C-peptide ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for C-peptide. The standards, controls, and samples are added to the microplate wells with the conjugate. The microplate is then incubated at room temperature on a microplate shaker at 700 to 900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. TMB Substrate is added, and the microplate is incubated a second time at room temperature on a microplate shaker at 700 to 900 rpm. Once the second incubation is complete, stop solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of C-peptide in the sample.