STEMdiff™ Monocyte Kit

For differentiation of human ES and iPS cells to monocytes.

Differentiate human pluripotent stem cells (hPSCs) to monocytes expressing CD14 with the feeder-free and serum-free STEMdiff™ Monocyte Kit. The simple protocol is performed in 2D adherent cultures. During the first 3 days, Medium A induces cells toward mesoderm. For the subsequent 4 days, mesodermal cells are further differentiated toward the hematopoietic lineage using Medium B. At Day 7, the medium is changed to Monocyte Differentiation Medium, which facilitates the differentiation to monocytes. CD14+ monocytes can be harvested directly from the culture supernatant starting as early as Day 14 and can be repeatedly harvested during the rest of the culture period. Peak CD14+ frequency is typically 60 to 80%.

The hPSC-derived monocytes can be further differentiated to dendritic cells or macrophages using the ImmunoCult™ Dendritic Cell Culture Kit or the ImmunoCult™-SF Macrophage Medium, respectively. For your convenience, components required for the preparation of the Monocyte Differentiation Medium, StemSpan™ SFEM II and STEMdiff™ Monocyte Differentiation Supplement (100X), are also available for purchase individually.

Caution: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').