Serum- and BPE-free medium for expansion of primary human airway epithelial cells.

PneumaCult™-Ex plus medium is a defined, serum- and BPE-free cell culture medium that supports more expansion of primary human airway and nasal epithelial cells at each passage, compared to other commercially available expansion media. This medium also supports at least two additional passages of cell expansion with better differentiation potential, defined as the ability to form a pseudostratified mucociliary epithelium at the air-liquid interface (ALI) using PneumaCult™-ALI medium or a cuboidal epithelium using PneumaCult™-ALI-S medium. PneumaCult™-Ex plus and either PneumaCult™-ALI or PneumaCult™-ALI-S constitute a fully integrated BPE-free culture system for in vitro human airway modeling. This robust and defined system is a valuable tool for basic respiratory research, toxicity studies, and drug development.