Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits.

• The kit purifies various target biomolecules via the formation of strong streptavidin-biotin bond
• Capture: Streptavidin
• Cleavage: C-terminal side of lysine and arginine amino acids
• Enzyme: Trypsin
• For use with (Application): Peptide quantitation

These kits were designed to remove the challenges often associated with the process of biomarker quantitation by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight.

The need to improve the ability to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging. As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility.

In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.